Effect of Sperm Chromatin Integrity on Parameters of In Vitro Fertilization

نویسندگان

  • Lubna H. Tahtamouni
  • Rania A. Wahdan
  • Randa M. Bawadi
  • Ali Z. Elkarmi
چکیده

صخلملا Infertility affects 15% of couples seeking children worldwide and male factor is evident in about 30-50% of the cases. To assess the effect of sperm chromatin integrity on the outcomes of intracytoplasmic injection, sperm DNA compactness was evaluated by acridine orange staining in 40 fertile and 44 infertile men attending one infertility clinic. The results showed no significant difference in the percentage of sperm with integrated DNA between the fertile and the infertile groups (p>0.05). Also, no significant difference (p>0.05) between fertile and infertile men was found in terms of fertilization rate, and pregnancy rate. However, a significant difference was found in developmental rate (p<0.05). When the percentage of sperm with DNA fragmentation was 38%, there was a decline in embryos quality. The current work concludes that conventional semen analysis is moderately predictive of an individual’s infertility. Thus, a need arises for new assays for the evaluation of sperm quality. ،ايملاع غلبت ةبسن جاوزلأا نيبغارلا باجنلإاب و نيزجاعلا نع قيقحت كلذ ببسب ىدحإ لكاشم مقعلا 15 % ، ثيح نوكي لجرلا لاوؤسم نع 30 50 % نم لثم هذھ تلااحلا . فدھت هذھ ةساردلا ىلإ سايق لدعم رسكت ةداملا ةيثارولا تاناويحلل ةيونملا ةدوجوملا يف تانيع لئاسلا يونملا عبرلأ و نيعبرا لجر ميقع و نيعبرلأ لجر بيصخ ) ءانب ىلع ليلحتلا يديلقتلا لئاسلل يونملا ( ، لذو ك مادختسإب ةقيرط ةغابص تاناويحلا ةيونملا ةدامب نيدركلأا ةيلاقتربلا . امك اھنأ فدھت ىلإ مييقت ريثأت رسكتلا يف ةداملا ةيثارولا ناويحلل يونملا ىلع تاجرخم ةيلمع نقحلا يرھجملا ناويحلل يونملا لخاد ءاشغلا يمزلابلا ،ةضيوبلل ةنراقمو لدعم هذھ تاجرخملا نيب صاخشلأا علا نيميق نيبيصخلاو . تلصوت ةساردلا ىلإ هنأ لا دجوي قرف يونعم نيب لاجرلا نيميقعلا نيبيصخلاو نم ثيح لدعم رسكت ةداملا ةيثارولا تاناويحلل ةيونملا وأ لدعم باصخلإا ،لمحلاو و نكل دجوي قرف يونعم نيب نيتعومجملا يف لدعم روطتلا ينينجلا . امك تدجو ةساردلا اضيأ هنأ يف لاح ة نوك ةبسن تاناويحلا ةيونملا تاذ ةداملا ةيثارولا ةرسكتملا ≤ 38 % نإف كلذ رثؤي ىلع ةيعون ةنجلأا ةجتانلا نم نقحلا يرھجملا كلذو ةدايزب دادعأ ةنجلأا ةطسوتم ةدوجلا ةئيدرلاو . نإ ليلحت لئاسلا يونملا ةقيرطلاب ةيديلقتلا دق لا نوكي اذ ةميق هدامتعلإ عجرمك رايتخلإ حلا ناوي يونملا بسانملا نم لجأ ةيلمع نقحلا يرھجملا لخاد ءاشغلا يمزلابلا ةضيوبلل . كلذل نإف ليلحت لدعم رسكت ةداملا ةيثارولا يف ناويحلا يونملا ربتعي ىرخأةميق ةلاعف . © 2009 Jordan Journal of Biological Sciences. All rights reserved

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تاریخ انتشار 2010